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2.4 DNA extraction and 16S rRNA gene sequencing Fecal samples were collected at the baseline and 16 weeks after treatment using the Longsee Fecalpro kit (Longsee Medical Technology Co., Guangzhou, China)

Lastly, it is crucial to identify patient groups likely to benefit from cuproptosis-based therapies, as certain cancers characterized by higher mitochondrial respiration, stem cell-like traits, or drug resistance have demonstrated better responsiveness to such treatments